抗生素检测


林可霉素,是林可链霉菌产生的林可酰胺类抗生素,作用于敏感菌核糖体,通过与50S亚基23SrRNA基因的中心环相结合,阻止肽链的延长,从而抑制细菌细胞的蛋白质合成。


名称 CAS号 英文名称 简称 母离子m/z 子离子m/z(a为定量子离子) 内标物
林可霉素 7179-49-9 Lincomycin LK 407.2 126.2a/359.3 盐酸强力霉素-D3

标准曲线

抗生素检测

y =0.05362 x + 0.12626 (r = 0.99897)


本底图谱


Sample Name Analyte Peak Name Area (cps) S/N Analyte RT(min) Target conc. () Calculated Concentration (ng/mL)
1 LK_1 2.311e3 8.9 3.43 N/A <0

本底图谱1

Sample Name Analyte Peak Name Area (cps) S/N Analyte RT(min) Target conc. () Calculated Concentration (ng/mL)
2 LK_1 1.968e3 9.5 3.42 N/A <0

本底图谱2

Sample Name Analyte Peak Name Area (cps) S/N Analyte RT(min) Target conc. () Calculated Concentration (ng/mL)
3 LK_1 1.926e3 5.1 3.17 N/A <0

本底图谱3

低标(加标100ng/L)图谱


Sample Name Analyte Peak Name Area (cps) S/N Analyte RT(min) Target conc. () Calculated Concentration (ng/mL)
4 LK_1 4.678e7 28728.3 3.41 N/A 108.38

低标(加标100ng/L)图谱1

Sample Name Analyte Peak Name Area (cps) S/N Analyte RT(min) Target conc. () Calculated Concentration (ng/mL)
5 LK_1 4.516e7 47375.1 3.42 N/A 113.72

低标(加标100ng/L)图谱2

Sample Name Analyte Peak Name Area (cps) S/N Analyte RT(min) Target conc. () Calculated Concentration (ng/mL)
6 LK_1 4.600e7 26524.2 3.42 N/A 114.36

低标(加标100ng/L)图谱2

高标(加标100ng/L)图谱


Sample Name Analyte Peak Name Area (cps) S/N Analyte RT(min) Target conc. () Calculated Concentration (ng/mL)
7 LK_1 7.998e7 40623.2 3.42 N/A 219.18
高标(加标100ng/L)图谱1

Sample Name Analyte Peak Name Area (cps) S/N Analyte RT(min) Target conc. () Calculated Concentration (ng/mL)
8 LK_1 8.190e7 59099.7 3.42 N/A 217.99

高标(加标100ng/L)图谱2

Sample Name Analyte Peak Name Area (cps) S/N Analyte RT(min) Target conc. () Calculated Concentration (ng/mL)
9 LK_1 7.941e7 44537.4 3.42 N/A 208.91
高(加标100ng/L)图谱3

低标加标回收率(范围 50%-200%):112.15%

高标加标回收率(范围 50%-200%):107.68%

低标精密度(3次重复标准偏差):2.93%

高标精密度(3次重复标准偏差):2.61%

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